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Kugelmeiers cell culture plates – Sphericalplate 5D (SP5D) - 6-Well Plate
Kugelmeiers cell culture plates – Sphericalplate 5D (SP5D) - 6-Well Plate

Kugelmeiers cell culture plates – Sphericalplate 5D (SP5D) - 6-Well Plate

P/N: 549-16010-00

The spherical plate 5D (SP5D) is a 3D cell culture plate for the formation of uniform, standardized spheroids with high quality and yield. It allows the effortless scaling and translation into the diagnostic or clinical application. At the same time, the reproducibility of follow-up tests increases, since the experiments always start under identical initial conditions due to the low size variance of the cultivated spheroids.

The patented geometry with special coating allows the integration of every single cell into a 3D-agglomerate in a controlled way. This is how uniformly sized spheroids grow without the formation of necrotic cores. According to the size and scale of the experiments, you can choose between our 24-well plate or our 6-well plate.

For small batches, we suggest using the 24-well plate containing 12 wells with 750 microwells each. Here, you will be able to yield up to 9,000 uniform spheroids. Furthermore, you can comfortably carry out monolayer comparative studies in the remaining 12 wells of the same plate.

The 6-well plate is suitable for scale-up trials and bigger batches. You can expect a yield of 3,364 spheroids per well, thus a total yield of 20,184 spheroids per plate.

The ready to use SP5D is particularly user-friendly: The cultivation does not require any pretreatment or centrifugation steps after seeding your cells. The media change is also particularly convenient due to simple pipetting, since the height of the microwells was chosen so that the cell clusters are retained. Moreover, the platform is compatible with standard automation units.

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Knowledge & Downloads

Technical Manual 6-Well-Plate
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SOP Manual 6-Well-Plate
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