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Whitepaper

Optimal system configuration for PFGE

December 2020

Optimal system configuration for PFGE
  • Bioanalytics
  • Pumping

Pulsed-field gel electrophoresis (PFGE) is a special gel electrophoresis type for the separation of DNA macromolecules. Due to the long runtimes and the sometimes high voltages, a constant and low buffer temperature is crucial for the success of the method. These low temperatures and long runtimes present a special challenge for the tubes used for the transport of the buffer solution.

In this whitepaper we evaluated the optimal peristaltic pump configuration for PFGE setups.

Heidolph Author: Heidolph

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Used Products

Hei-FLOW Core 120 Multi

Hei-FLOW Core 120 Multi

Para aplicaciones y transferencia de líquidos estándar
SP quick (ep 1,6 mm)

SP quick (ep 1,6 mm)

Para tubos con un espesor de pared (ep) de 1,6 mm
PharMed® - espesor de pared: 1,6 | d. int.: 0,8 | d. ext.: 4 (por metro)

PharMed® - espesor de pared: 1,6 | d. int.: 0,8 | d. ext.: 4 (por metro)

Ideal para usos médicos, de laboratorio y de investigación