Whitepaper
Optimal system configuration for PFGE
December 2020
- Bioanalytics
- Pumping
Pulsed-field gel electrophoresis (PFGE) is a special gel electrophoresis type for the separation of DNA macromolecules. Due to the long runtimes and the sometimes high voltages, a constant and low buffer temperature is crucial for the success of the method. These low temperatures and long runtimes present a special challenge for the tubes used for the transport of the buffer solution.
In this whitepaper we evaluated the optimal peristaltic pump configuration for PFGE setups.
Author: Heidolph
